Analysis of CXCR4 expression in prostate cancer cell lines stably overexpressing SLUG. (A-D) qPCR and RT-PCR analysis of RNA transcripts of CXCR4 expression. RNA was extracted from PC3 (A), DU145 (B), 22RV1 (C), and LNCaP (D) cell lines stably carrying pMIGR1-Slug or vector control (pMig), and subjected to cDNA synthesis. The transcript level of CXCR4 was analyzed by qPCR (left panel) and RT-PCR (right panel). GAPDH was included as a control. CXCR4 was highly expressed in PC3, DU145, 22RV1, and LNCaP cell lines overexpressing SLUG. (E) Western Blot analysis of CXCR4 expression in PC3, DU145, 22RV1, and LNCaP cell lines stably carrying pMig-Slug or pMig (vector). GAPDH was included as a loading control.