Figure 5

Overexpression of Slug positively regulates MMP9 expression in prostate cancer cell lines. (A, B) Analysis of MMP9 RNA transcripts in prostate cancer cell lines overexpressing SLUG by qPCR (left panel) and RT-PCR (right panel). RNA was extracted from PC3 and DU-145 cell lines stably carrying pMig-Slug or pMig (vector) and then subjected to cDNA synthesis. Transcript level of MMP9 was analyzed by qPCR and RT-PCR. GAPDH was included as a control. MMP9 was highly expressed in PC3 and DU145 overexpressing SLUG. (C, D) Zymographic analysis of MMP9 activity in prostate cancer cells overexpressing SLUG. Culture medium were collected from PC3 (C) and DU145 (D) cell lines stably carrying pMig or pMig-Slug and then assayed for MMP9 activity by gelatin-zymography. The left and right panels are gel image and relative MMP9 activity based on density of bands, respectively.