Figure 6

Immunohistochemical analysis of tumor tissues for cell proliferation and tumor vasculature. A, IHC staining to determine tumor vasculature and proliferation was performed in 5 μm sections on liver. Samples from animals treated with 10 μ g transposon plasmid and varying amounts of transposase plasmids were stained using anti-CD31 (tumor vasculature) and anti-Ki67 antibodies (tumor proliferation) and visualized by light microscopy. B, Percent positively staining area using Ki67 antibody; C, Percent positively staining area using CD31 antibody. Percentage of positively stained areas in respective groups was determined by measuring the area of immunopositivity vs. total tissue area in each of 10 random fields (mean +/- SD). Transposon (pKT2/BidAEL), and transposase (pPGKSB11) plasmid DNA amounts infused into animals are summarized below the graph. *P < 0.05; **P < 0.005