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Figure 3 | Molecular Cancer

Figure 3

From: Papillomavirus E5: the smallest oncoprotein with many functions

Figure 3

BPV E5 and PDGFβ-receptor co-precipitate and co-localize in bovine urinary bladder carcinoma. A. Co-immunoprecipitation of the PDGFβ-receptor with E5 antiserum. Tissue lysates were immunoprecipitated with the anti E5 antibody and the immunoblot was analyzed with the anti- PDGFβ-receptor antibody. Lanes 1-3 are BPV-2 positive bovine urinary bladder cancer samples, T1, T2, T3. Lane 4 is BPV-2 DNA positive normal bovine bladder mucosa, N1. B. Coimmunoprecipitation of the E5 oncoprotein with the PDGFβ-receptor antiserum. The arrow indicates the E5 oncoprotein and its estimated kDa weight. In a parallel blot, the same amount of lysate was probed with an antibody to β-actin to control for the quantity of protein (bottom panel) C. Colocalization (yellow) of PDGFβ-receptor (red) and E5 (green) in bovine neoplastic urothelium. The white arrows indicate a juxtanuclear position of E5 and PDGFβ-receptor (reproduced with permission from Borzacchiello et al., Oncogene 2006).

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