Figure 6

Expression changes of intronic lncRNAs mapping to PPP3CB , MAP3K14 and DAPK1 loci are not accompanied by changes in the corresponding protein-coding mRNAs. Relative levels of intronic lncRNAs and protein-coding mRNAs from PPP3CB(A), MAP3K14(B) and DAPK1(C) loci were determined by Real-Time PCR in clinical samples of primary adenocarcinoma (circles) or distant metastasis (squares) from pancreatic cancer patients. For each transcript, the number of tested samples is indicated in the × axis. For each gene, results are expressed as fold-change relative to the average expression of primary tumor samples. For each sample, qPCR assays were performed in triplicate and mean values are shown. House-keeping gene HMBS was used as the endogenous control for normalization across patient samples. All intronic transcripts (left panels) were differentially expressed in metastatic samples at a significance threshold of p< 0.05 (PPP3CB, p= 0.0259; MAP3K14, p= 0.0069; DAPK1, p= 0.0035). Protein-coding transcripts (right panels) were not significantly differentially expressed.