Figure 2
From: Anti-tumor effects of fibroblast growth factor-binding protein (FGF-BP) knockdown in colon carcinoma
Effects of FGF-BP expression on cell proliferation and colony formation. Clonal or mass-transfected shRNA expressing LS174T cell lines with reduced FGF-BP levels show decreased anchorage-dependent cell proliferation as determined in WST-1 assays (A). Anti-proliferative effects in LS174T cells are dependent on the extent of RNAi-mediated FGF-BP knockdown, and the correlation between residual FGF-BP levels and the proliferative activity establishes an FGF-BP 'gene-dose effect' on cell proliferation (B). Soft-agar assays reveal marked reductions of anchorage-independent proliferation upon FGF-BP knockdown in LS-174T (C) and in HT29 cells (D). Anti-proliferative effects are confirmed in HCT-116 cells that show decreased anchorage-dependent (E, left) and anchorage-independent proliferation (E, right) upon FGF-BP knockdown.