Figure 3
From: Anti-tumor effects of fibroblast growth factor-binding protein (FGF-BP) knockdown in colon carcinoma
Effects of FGF-BP expression on cell cycle and apoptosis. Stable FGF-BP knockdown cells vs. control LS174T cells were treated with nocodazole 20 h prior to propidium iodide/FACS cell cycle analysis. Decreased percentages of cells in G2/M arrest demonstrate slower cell cycle progression after FGF-BP knockdown (A), while sub-G0 levels are increased (A; see also right bar diagram). Caspase-3/7 assays show the induction of apoptosis, represented by increased caspase-3/7 activity, upon FGF-BP knockdown in LS174 (B, left) or HCT-116 cells (B, right). Expression of exogenous FGF-BP leads to anti-apoptotic effects in endogenously FGF-BP-negative SW13 cells (C, right), but not in LS174T cells with already high endogenous FGF-BP levels (C, left).