Figure 8

Co-regulation of NONO and YB-1 in SW480 cells. A) Basal expression of YB-1, NONO, and RALY in SW480 and HT29 cells. B) Histogram representing the relative expression of YB-1, NONO, and RALY in untransfected cells. Error bars represent the standard error of the mean. C) Growth curves of SW480 and HT29 cells. Cells were plated in 35 mm petri dishes and live cells were counted every other day with a hemacytometer. D) SW480 cells were transfected with siRNA sequences against NONO (siNONOa) or RALY (siRALYc) and 96 hours later cell lysates were extracted for Western analyses with antibodies against NONO, RALY, and YB-1. E) Histogram representing the relative expression of YB-1 in cells transfected with siNONOa or siRALYc. Error bars represent the standard error of the mean. F) SW480 cells were transfected with three different siRNA sequences against YB-1 and 96 hours later cell lysates were extracted for Western analyses with antibodies against NONO, RALY and YB-1. In every blot, β-actin was used as loading controls.