Figure 3
From: Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity
Cathepsin S surface expression quantification on cancer cell lines. (A) Cathepsin S surface expression on Colo205, LoVo, Bx-PC3, Aspc-1 and Panc-1 by flow cytometry. (B) QuantiBRITE PE beads fluorescence quantification of cathepsin S expressed on the surface of cathepsin S-positive cell lines (Colo205, LoVo, BxPC-3 and Aspc-1). (C, D) Confocal microscope images of LoVo (C) and Aspc-1 (D) cell lines show human tumor expression of cathepsin S on the cell surface (Colo205, BxPC-3 and Panc-1 shown in supplementary figures): Left panels show isotype control while right panels show cathepsin S specific antibody (green) binding to the cell surface, indicated with arrow heads.