Histological analysis of markers genes. Protein expression was determined by immunohistochemistry using sections from Formalin-fixed paraffin-embedded tissue (A: MUC1, B: SPP1, C: RRM2, D: FOXM1, E: DEPDC1, F: NUSAP1). For each protein, expression is shown in human breast tissue with a rising degree of malignancy (healthy, DCIS, invasive breast tumour). Specific signals are represented by pink staining (arrowhead) (counterstained with haematoxylin, original magnification 400×, bars:100 μm). The inserts depict the negative controls as a reference.