Figure 2

Cardiac glycosides preferentially target GBM cells over non tumor astrocytes (NTAs): U-87, D54 GBM cells and NTA cells when treated with different concentrations of cardiac glycosides demonstrated a significantly higher cytotoxicity (p < 0.05) against GBM cells at concentrations ranging from 50 μM to 500 nM. At concentrations of 100 nM and below there was no significant cytotoxic effect. A: Bar graph demonstrating preferential cytotoxicity of Digoxin against U-87 and D54 GBM cells B: Bar graph demonstrating preferential cytotoxicity of Ouabain against U-87 and D54 GBM cells. C and D: Growth curves of U-87 GBM and NTAs were plotted for four days using an alamarBlue based assay demonstrate preferential inhibition of U-87 GBM cells compared to NTAs when treated with 500 nM of Digoxin (2C) and Ouabain (2D).