Figure 1

TSA induces ATX expression in various cancer cell types. A, SW480 cells were treated with Aza (5 μM) for 48 hrs or TSA (100 nM) for 24 hrs, followed by ATX mRNA detection by RT-PCR. B, SW480 cells were treated with the indicated concentrations of TSA for 24 hrs. The ATX mRNA expression in cells and ATX protein in the conditional medium were detected by RT-PCR and Western blotting analyses, respectively. C, SW480 cells were treated with TSA (100 nM) for 0, 6, 12, or 24 hrs. After TSA treatment, the ATX mRNA expression in cells and secreted ATX protein in the conditional medium were examined by real-time RT-PCR and Western blotting analyses, respectively. D, LysoPLD activity in the conditional medium was determined with FS-3 as substrate after TSA treatment for indicated times. E, SW480 cells were treated with VPA (1 mM), NaB (1 mM), or TSA (100 nM) for 24 hrs. The ATX mRNA expression was evaluated by real-time RT-PCR. F. The indicated breast, cervical, lung, ovarian, and prostate cancer cell lines were treated with or without TSA (100 nM for 24 hrs), and then ATX mRNA expression levels were detected by RT-PCR. The p values derived from Student's t test are (*) p < 0.005, (**) p < 0.001.