Figure 5
The TSA-induced ATX protects cancer cells from TSA-induced apoptosis through cell specific LPA receptor-mediated signaling. A, The mRNA expression levels of LPA1, LPA2 and LPA3 in SW480 cells were examined by real-time RT-PCR. SW480 cells were transfected by LPA2 siRNA with non-specific siRNA (siNC) as control. After siRNA transfection for 48 hrs, SW480 cells were treated with or without TSA (250 nM) in the presence of LPC (100 μM) or LPA (5 μM). The apoptosis of SW480 cells were measured after TSA treatment for 24 hrs. B, The mRNA expression levels of LPA1, LPA2 and LPA3 in MDA-MB-231 cells were examined by real-time RT-PCR. MDA-MB-231 cells were transfected by ATX or LPA1 siRNA with non-specific siRNA (siNC) as control. After siRNA transfection for 48 hrs, MDA-MB-231 cells were treated with or without TSA (250 nM) in the presence of LPC (100 μM). The apoptosis of MDA-MB-231 cells was measured after TSA treatment for 24 hrs. The p value derived from Student's t test is (**) p < 0.001.