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Figure 4 | Molecular Cancer

Figure 4

From: Response to mTOR inhibition: activity of eIF4E predicts sensitivity in cell lines and acquired changes in eIF4E regulation in breast cancer

Figure 4

eIF4E activities vary in different cell lines. A) Cells were transiently transfected with equal copy numbers of plasmids to allow expression of transcripts with the GFP reading frame preceded by either a control 5'UTR lacking regulatory motifs (con) or the eIF4E-responsive structured 5'UTR (struc). GFP protein and mRNA were quantified by flow-cytometry and real-time PCR respectively. Translational efficiency (protein synthesised per unit mRNA) is presented relative to the control. Data points represent means (+/-standard deviations) of values from three separate wells of cells from two independent experiments (a total of six values). B) Translational efficiencies of transcripts with eIF4E-responsive 5'UTRs in the 9 cells lines.

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