Figure 1
From: Efficient TGF-β/SMAD signaling in human melanoma cells associated with high c-SKI/SnoN expression
SKI and SnoN expression in human melanoma cell lines. A. Total protein extracts (40 μg) from unstimulated cultured melanoma cell lines and normal melanocytes were analyzed by Western blotting for SKI, SnoN and P-SMAD3 levels. An anti-actin antibody served for normalization. B. SKI mRNA steady-state levels, as measured by quantitative RT-PCR. C. Western analysis of the ubiquitin E3 ligases Arkadia and Smurf2 levels in the same cell extracts as in panel A. D. 1205Lu at approx. 80% confluency were incubated for 8 h with MG132 (10 μM) before protein extracts were prepared from cell lysates and processed for Western analysis of SKI content.