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Table 2 SMAD7 overexpression alters 1205Lu melanoma cell tumorigenicity and metastatic potential without altering SKI levels.

From: Efficient TGF-β/SMAD signaling in human melanoma cells associated with high c-SKI/SnoN expression

1205Lu clones

SKI protein levels (Western blotting)

Relative attenuation of TGF-β-dependent SMAD3/4-specific transcriptional response

Matrigel™ invasion

Subcutaneous tumor growth in mice

Bone metastases in mice

Pc

100

0

100

600

6/6 - 10/11

SMAD7a

82

70

55

nd

1/7 - 0/12

SMAD7b

107

50

45

nd

0/7 - 0/12

  1. SKI protein levels in mock (pc) or SMAD7-overexpression 1205Lu cell populations (SMAD7a and SMAD7b) were determined by scanning densitometry of corresponding Western analyses. Matrigel™ invasion results are expressed as % of the values obtained with mock-transfected cells. Values for subcutaneous tumor growth in mice represent the average tumor volume 40 days after injection of tumor cells (mm3). Values for bone metastases in mice represent the ratio of mice bearing osteolytic bone metastases vs. total number of mice in each group at 5 weeks post-inoculation of tumor cells in 2 separate experiments. Details about experimental procedures may be found in [36, 37]. nd: not detectable.
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Molecular Cancer

ISSN: 1476-4598