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Figure 2 | Molecular Cancer

Figure 2

From: Differential contributory roles of nucleotide excision and homologous recombination repair for enhancing cisplatin sensitivity in human ovarian cancer cells

Figure 2

NER efficiency of various cisplatin-sensitive and -resistant ovarian cancer cell lines determined by ISB. A2780 and CP70 (A, B), A2780 and CDDP (C, D), 2008 and 2008C13 (E, F), PEO1 and PEO4 (G, H) cells were pre-treated with HU for 24 h, then treated with various concentration of cisplatin for 1 h (A2780: 10 μM; CP70: 40 μM; CDDP: 40 μM; 2008: 7.5 μM; 2008C13: 40 μM; PEO1: 15 μM; PEO4: 20 μM), and further cultured in HU containing medium for the indicated time periods. Total DNA was isolated and analyzed by ISB assay for cisplatin-induced intrastrand cross-links with anti-Pt-GG antibody. The intensity of each band was quantified by scanning images and processing with Alphaimager-2000 software. The relative percentage of remaining Pt-GG at different time points is an average of three independent repeats. (n = 3, Bar: SD).

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Molecular Cancer

ISSN: 1476-4598