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Figure 3 | Molecular Cancer

Figure 3

From: Differential contributory roles of nucleotide excision and homologous recombination repair for enhancing cisplatin sensitivity in human ovarian cancer cells

Figure 3

DNA repair capacity of various cisplatin-sensitive and -resistant ovarian cancer cell lines determined by HCR. A2780 and CP70 cells (A), PEO1 and PEO4 cells (B) were transfected with cisplatin-damaged and undamaged pCMV-Tag 2 plasmid. As an internal control, the pGL4.73 plasmid, which carries a renilla luciferase gene, was co-transfected with the pCMV-Tag 2 plasmid. The cells were harvested 2 days after transfection, both firefly and renilla luciferase activities were determined. The activity of firefly luciferase in each experiment was calculated as relative activity to the renilla luciferase activity to minimize the experimental variations. The ratio of luciferase activities in the same cell line for both undamaged and damaged plasmid was used to determine the DNA repair ability of the host cells. The relative luciferase activity is an average of three independent repeats. Bars represent standard deviation (SD).

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