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Figure 3 | Molecular Cancer

Figure 3

From: Overexpression of Aurora-A in primary cells interferes with S-phase entry by diminishing Cyclin D1 dependent activities

Figure 3

Importance of known Aurora-A interactions for cell cycle inhibition. (A) Wi-38 cells were arrested in G0 by serum deprivation and infected with adenoviruses expressing Aurora-A or control virus expressing lacZ and analyzed by immunoblotting with the antibodies indicated. (B) Quiescent cells were infected with adenoviruses expressing the indicated proteins and analyzed by immunoblotting and PI staining at time points 0 and 26 hours after serum addition. (C) Serum arrested cells expressing Aurora-A or lacZ were transfected with the indicated siRNA 24 hours prior to serum addition. At the indicated time points cells were harvested and analyzed as in (B). (D) Serum deprived cells infected with control or Aurora-A expressing viruses were harvested at the indicated times and analyzed by immunoblotting with antibodies recognizing phosphorylation of ERK1/2 and ribosomal protein S6, respectively. For all illustrations, representatives of at least two independent experiments are shown.

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Molecular Cancer

ISSN: 1476-4598