Figure 2

miR-449 is part of the miR-34 family and inhibits cell proliferation. A - miR-449 is part of the miR-34 family and is evolutionarily conserved. B - miR-449 re-introduction into human gastric cell lines (SNU638) inhibits cell proliferation (red line) compared to a scrambled control (blue line) and miR-146 control (black line). Error bars represent S.D. C - Visual inspection of cell proliferation inhibition and senescence-like phenotype upon miR-449 re-introduction into SNU638 cells (lower panel) compared to scrambled transfection control (upper panel). D - FACS cell cycle analysis showing sub-G₁ accumulation of SNU638 cells 72 hours following miR-449 re-introduction (right histogram) compared to a scrambled transfection control (left histogram) The table shows cell accumulation in G₁ fraction upon miR-449 re-introduction compared to scrambled RNA control at 48 hours post transfection, followed by a shift to the sub-G₁ fraction at 72 hours post transfection indicative of cell death. E - Senescent phenotype of SNU638 cells upon miR-449 and miR-34a positive control re-introduction shown by acidic β-gal assay compared to RNA scrambled control.