Figure 1

TRAIL expression in the skin driven by the human keratin 14 promoter. (A) Diagram of the construct introduced into ES cells. Between the mouse TRAIL cDNA and the human keratin 14 promoter, one β-globin intron and small parts of exon 1 and exon 2 were placed. The 3'UTR of SV40 was added at the end the TRAIL cDNA. The primers used for RNA expression analysis and the probe used for in situ hybridization are indicated on the scheme. (B) RNA was extracted from skin of wild type and transgenic animals and after reverse-transcription, semi-quantitative PCR was performed using primers specific for the transgene. 36B4 was used as a positive control. (C) RNA expression was also evaluated by in situ hybridization, using a probe specific for the transgene. Blue staining shows TRAIL expression in the basal cell layer of epidermis of transgenic animals. Bar, 100 μm. (D) Western blot analysis of protein extracts from wild-type and transgenic mice using an anti-TRAIL antibody and actin antibody as a loading control.