Figure 4

CD11b⁺or CD68⁺TAM abundantly express VEGF-C/D and VEGFR-3. 1 × 10⁶ MBT-2 cells were injected into the urinary bladders of 8-10-week old female C3H mice. 4 weeks after the tumor cell injection, single cell suspension of OUBC was prepared. (A) CD11b⁺ cells and CD11b^- cells were enriched by FACS with ~95% purity. (B) Comparison of mRNA expressions of VEGF-C/D in CD11b⁺ cells. Data are presented as relative fold to CD11b^- cells after standardization with hypoxanthine-guanine phosphoribosyl transferase (HPRT). Graph shows mean ± SD; n = 3 for each group. *p < 0.05 versus CD11b^- cells. (C) Images showing CD11b⁺ or CD68⁺ TAM and VEGF-C⁺ or VEGFR-3⁺ cells in the sectioned samples. Scale bars, 50 μm.