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Figure 6 | Molecular Cancer

Figure 6

From: Soluble vascular endothelial growth factor receptor-3 suppresses lymphangiogenesis and lymphatic metastasis in bladder cancer

Figure 6

Depletion of CD11b+/CD68+TAM reduced OUBC-induced lymphangiogenesis and lymphatic spread of bladder cancer cells. 1 × 106 MBT-2 cells were injected into the urinary bladders of 8-10-week old female C3H mice. 2 weeks after the tumor cell injection, clodronate liposome (CDL) or control liposome (CL) were injected intraperitonealy to the OUBC mice once every 3 days. 4 weeks after the tumor cell injection, the bladder and SLN were sampled. (A) Images showing H&E stained sections of tumors. Scale bars, 2 mm. (B) Comparison of cross-sectional area of tumors. Graph shows mean ± SD; n = 5 for each group. (C) Images showing LYVE-1+ LVs and PECAM-1+ BVs in tumors. Scale bars, 200 μm. (D) Comparison of LV numbers at different sizes of LV in the tumor sections. Graph shows mean ± SD; n = 5 for each group. *p < 0.05 versus CL. (E) Comparision of BVD per total sectioned area of tumors. Graph shows mean ± SD; n = 5 for each group. (F) Left panel; images showing LYVE-1+ LVs and PECAM-1+ BVs in LNs. Right panel; images showing LYVE-1+ LVs and CytoK+ metastatic cancer cells in LNs. Scale bars, 500 μm. (G) Comparison of LVD and BVD in LNs. Graph shows mean ± SD; n = 5 for each group. (I) Comparison of densities of CytoK+ metastatic cancer cells in LNs. Graph shows mean ± SD; n = 5 for each group. *p < 0.05 versus CL.

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