Basal expression levels of Keap1, Nrf2 and Nrf2-regulated genes GCLC and AKR1c1 and GSH amongst a panel of pancreatic cancer cell lines. A, Immunoblot detection of basal Keap1 protein in MiaPaca-2, Panc-1, FAMPAC, Paca-2 and Suit-2 cells. B, Immunoblot detection of basal Nrf2 in cells untreated or treated with the proteosome inhibitor MG132 (10 μM) for 2 h in order to visualize Nrf2 protein. The band labelled 'non-specific' was not depleted following transfection with 10 nM Nrf2-targeting siRNA Nrf2 (data not shown). Beta actin was used as a reference control for blots A and B. C, Immunoblot detection of basal GCLC and AKR1c1. D, Total basal glutathione levels. * = P < 0.05,** = P < 0.01,*** = P < 0.001.