Response of Nrf2-target genes to siRNA depletion of Nrf2 in FAMPAC, MiaPaca-2 and Suit-2 pancreatic cancer cells. Cells were transfected with 10 nM Nrf2-targeting siRNA, or Stealth RNAi control, for 96 h. A, qRTPCR analysis of relative Nrf2 mRNA expression. GAPDH was used as a reference control. B, Immunoblot detection of GCLC and AKR1c1 in three parallel experiments. Beta-actin is used as reference control. C, Total glutathione levels. Data are the means ± S.D. of four discrete experiments. * = P < 0.05,** = P < 0.01.