Effect of siRNA depletion of Nrf2 on cell cycle progression and apoptosis status of Suit-2 and FAMPAC pancreatic cancer cells. Cells were transfected with 10 nM Nrf2-targeting siRNA, or Stealth RNAi control, for 72 h. A, Flow cytometric analysis of cell cycle status in propidium iodide stained cells. Data are the means ± S.D of four discrete experiments. * = P < 0.05. B, Micrographs depicting cells analysed in A. C, Flow cytometric analysis of apoptotic status in Annexin 5/propidium iodide stained cells.