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Figure 6 | Molecular Cancer

Figure 6

From: Berberine modulates AP-1 activity to suppress HPV transcription and downstream signaling to induce growth arrest and apoptosis in cervical cancer cells

Figure 6

Berberine induces apoptosis, activation of Caspase 3 and loss of mitochondrial membrane potential in cervical cancer cells. A, Flowcytometric analysis of SiHa and HeLa cells treated with berberine for 24 h. Treated cells were examined for apoptotic cells using Annexin V-FITC apoptosis detection kit. B, Immunoblot analysis of cellular protein isolated from SiHa and HeLa cells treated with berberine for PARP-1 expression as described in "Methods". C, Flowcytometric analysis of SiHa and HeLa cells for active caspase-3. Percentage in histograms shows proportion of cell with active caspase-3 after berberine treatment. D, Immunofluorescence photomicrograph of JC-1 stained untreated, control (DMSO only) and berberine treated SiHa and HeLa cells. Cells appearing in red channel indicate intact mitochondrial transmembrane potential, whereas cells appearing in green channel indicate loss of mitochondrial membrane potential.

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