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Figure 3 | Molecular Cancer

Figure 3

From: Dual induction of caspase 3- and transglutaminase-dependent apoptosis by acyclic retinoid in hepatocellular carcinoma cells

Figure 3

Induction of apoptosis in JHH-7 cells using Sp1 and EGFR siRNAs. A, JHH-7 cells overexpressing non-target or Sp1 siRNA were harvested and protein levels of Sp1, EGFR, and GAPDH determined by Western blotting. B, JHH-7 cells overexpressing non-target or 2 kinds of EGFR siRNAs were harvested and protein levels of EGFR and GAPDH determined by Western blotting. C, Numbers of viable cells were counted 2 days after seeding these cells on 35 mm dishes. Results shown are the number of viable cells relative to the controls, expressed as % ± S.D. **P < 0.01 compared to control cells. D, The seeded cells on cover slips in 35 mm dishes were fixed and stained with Hoechst (upper panels) and TUNEL (second panels). Panels A-D show representative results from 3 different experiments with similar results.

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