Figure 4

Nuclear accumulation of TG2 and CLSp1 observed in the liver of nude mice transplanted with JHH-7 cells, and in DEN-treated rats with liver cancer after ACR treatment. A, Liver sections including normal (panels D-F and J-L) and metastatic areas (panels A-C and G-I) from JHH-transplanted nude mice following treatment with ACR were stained with polyclonal anti-TG2 (30 μg/ml; panels A, D), anti-CLSp1 (30 μg/ml; panels B, E), anti-cleaved caspase 3 (10 μg/ml; panels C, F), anti-EGFR (10 μg/ml; panels G, J), and non-immune antibodies (NI IgG; 30 μg/ml; panels H, K). B, Liver sections from normal and neoplastic areas in DEN-treated rats following treatment with vehicle or ACR (at 40 and 80 mg/kg) were stained as in Figure 4A. The signals were enhanced with an ABC kit and developed with DAB substrate. Sections were counterstained with hematoxylin-eosin (HE; Figure 4A, panels I, L, and Figure 4B, bottom panels). Arrows indicate signals under the levels for each antigen. Scale bar, 50 μm.