Figure 4

Effects of DZNeP on invasion and in vivo tumor growth. A, Cells were treated with DZNeP (1 μM, 3 days) and assayed for cell viability at the end of the treatment (trypan blue staining). Alive cells were used for Matrigel invasion assay, as described in "Materials and Methods". Columns, mean volume; bars, standard deviation. **p < 0.01 with respect to untreated cells (T test). B, mRNA levels in invading and non-invading cells (QT-PCR). C, effects of DZNeP on LNCaP cell tumorigenicity. **p < 0.01 (log rank test) with respect to untreated cells. D, effects of DZNeP on DU145 xenograft tumor volume. *p < 0.05 with respect to untreated cells (U test). Star colors refer to dose treatment (blue, 1 μM; red, 10 μM). Number of animals: untreated: 6; 1 and 10 μM: 8. Point, mean value; bar, standard deviation.