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Figure 2 | Molecular Cancer

Figure 2

From: HBx Sensitizes Cells to Oxidative Stress-induced Apoptosis by Accelerating the Loss of Mcl-1 Protein via Caspase-3 Cascade

Figure 2

HBx enhances production of cellular ROS and sensitizes hepatocytes to H 2 O 2 -induced apoptosis. (A) Indicated cells were treated with or without H2O2 (400 μM) for 15 hr and then incubated with DCFH-DA for 30 min and the production of ROS was determined. Representative results are shown. Magnification, ×100. (B) Cells were exposed to the indicated amount of H2O2 for 15 hr and the protein levels of PARP, caspase-3, HBx and GAPDH were determined by Western blot assay. "ns", nonspecific bands. (C) HepG2 cells were infected with Ad-HBx-myc or Ad-blank at the indicated MOI for 36 hr followed by treated with H2O2 (400 μM) for additional 15 hr. Expression of PARP, caspase-3, Myc-tag and GAPDH was analyzed by Western blot assay. (D) HepG2.2.15 or HepG2 cells were treated with or without H2O2 (400 μM) for 15 hr and protein levels of PARP, caspase-3 and GAPDH were measured by Western blot assay. (E) SMMC-7721 cells were transiently transfected with p3.8II or p3.8IIxm for 36 hr followed by treated with or without H2O2 (400 μM) for additional 15 hr. Then cell lysates were subjected to Western blot and probed with indicated antibodies.

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