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Figure 3 | Molecular Cancer

Figure 3

From: Role of bile salt in regulating Mcl-1 phosphorylation and chemoresistance in hepatocellular carcinoma cells

Figure 3

GCDA induces Mcl-1 phosphorylation at T163 site, which is essential for GCDA-stimulated survival. (A) Huh7 cells overexpressing exogenous HA-tagged WT, T163A Mcl-1 mutant or vector-only control cells were metabolically labeled with 32P-orthophosphoric acid and treated with GCDA for 30 min. Mcl-1 was immunoprecipitated using an HA antibody. Phosphorylation of Mcl-1 was determined by autoradiography. (B) and (C) Huh7 cells expressing WT or T163A Mcl-1 mutant were treated with cisplatin (30 μM) or irinotecan (40 μM) in the absence or presence of GCDA (100 μM) for 48 h. Cell viability was determined by analyzing annexin-V binding by FACS. Data represent the mean ± S.D. of three separate determinations.

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