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Figure 6 | Molecular Cancer

Figure 6

From: Role of bile salt in regulating Mcl-1 phosphorylation and chemoresistance in hepatocellular carcinoma cells

Figure 6

Mcl-1 is essential for GCDA-induced chemoresistance in HepG2 cells. (A) Mcl-1 siRNA (15 nM) or control siRNA (15 nM) was transfected into HepG2 cells using Lipofectamine 2000. After 24 h, the levels of Mcl-1 expression were analyzed by Western blot. (B) and (C) HepG2 cells expressing Mcl-1 siRNA or control siRNA were treated with cisplatin (30 μM) or irinotecan (40 μM) in the absence or presence of GCDA (100 μM) for 24 h. Cell viability was determined by analyzing annexin-V binding by FACS. Data represent the mean ± S.D. of three separate determinations.

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