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Figure 4 | Molecular Cancer

Figure 4

From: High susceptibility of metastatic cells derived from human prostate and colon cancer cells to TRAIL and sensitization of TRAIL-insensitive primary cells to TRAIL by 4,5-dimethoxy-2-nitrobenzaldehyde

Figure 4

Over-expression of c-Myc and the cross-talk between DNA-PKcs and c-Myc in metastatic cancer cells. (A) The levels of c-Myc and DNA-PKcs proteins in PC3 and PC3-MM2 cells (left) and KM12 and KM12L4A cells (right) were determined using Western blot analysis. (B) Immunoprecipitates (IP) of the DNA-PKcs or c-Myc antibody from PC3 and PC3-MM2 cells were subjected to Western blot analysis with c-Myc, phospho-c-Myc (pMyc) or DNA-PKcs antibody to determine the interaction between DNA-PKcs and c-Myc. IgG was used as an internal control for the immunoprecipitation. (C) PC3 cells were transfected with siRNA against DNA-PKcs or scrambled siRNA as a control. After 48 h, the cell lysates of the transfectants were subjected to Western blot analysis with c-Myc or pMyc antibody.

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