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Figure 4 | Molecular Cancer

Figure 4

From: MDM2 antagonists boost antitumor effect of androgen withdrawal: implications for therapy of prostate cancer

Figure 4

Role of AR reduction in apoptotic response of LNCaP cells to nutlin. A) Knockdown of AR in LNCaP cells by siRNA. siRNA (50 nM) was transfected in LNCaP cells and 24 h later 5μM Nutlin-3a was added for another 24 h before Western analysis B) AR knockdown increased apoptotic response to nutlin. Following 24 h knockdown of AR, LNCaP cells were washed and subsequently treated with 5 μM Nutlin-3a for a an additional 48 h and analyzed for Annexin V ± SD. C) AR knockdown does not increase further nutlin-induced apoptosis in CSS. LNCaP cells were transfected with siRNA for 24 h. Cells were then washed with PBS and maintained in CSS for an additional 48 h in the presence or absence of nutlin and apoptotic fraction measured as above.

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