Skip to main content
Figure 6 | Molecular Cancer

Figure 6

From: MDM2 antagonists boost antitumor effect of androgen withdrawal: implications for therapy of prostate cancer

Figure 6

Nutlin-3a greatly enhances antitumor activity of androgen deprivation in vivo. A) LNCaP cells were grown subcutaneously in pre-castrated male athymic nude mice in the presence of testosterone pellets until reaching approximately 400 mm3 on average. Mice were administered Nutlin-3a at 200 mg/kg bid (n = 10) or testosterone pellets were removed to simulate androgen ablation (n = 20). Additional mice were treated with a combination of ablation + Nutlin-3a at 200 mg/kg bid orally for 14 days (n = 20). Control mice were dosed with vehicle for 14 days + sham pellet removal under anesthesia (n = 10). B) PSA levels reflect changes in tumor volume. At day 42, five random mice in each group were bled and PSA levels ± SD were determined. C) Combination of nutlin and androgen deprivation increases lifespan of mice. After completion of treatment, mice were monitored for over a year and ILS was calculated by the Kaplan-Meier formula. Survival was calculated using a cut-off of 1000 mm3.

Back to article page