Figure 1
From: Mutation of NIMA-related kinase 1 (NEK1) leads to chromosome instability
Genomic instability in NEK1 deficient cells. A. Renal tubular epithelial cells from wild type and NEK1/kat2J -/- mice were fixed with 4% paraformaldehyde during exponential phase growth and stained with DAPI for nuclear morphology Almost all wild type cells had normal nuclear morphology (panel a). NEK1 -/- cells often had micronuclei (panel b, small arrows), multiple nuclei (panel c, arrows), or hollow nuclei (panel d, arrow heads). Chromosome bridges were also frequently observed (panel b, large arrow). B. Each histogram represents percentages (means ± standard errors) of each type of abnormal nuclei from 2 distinct cell lines at passage 4, and totals of 1000 cells scored per cell line.