Figure 5
From: Mutation of NIMA-related kinase 1 (NEK1) leads to chromosome instability
Cultured NEK1 -/- cells form colonies in soft agar and tumors in when injected into mice. A, B. Equal numbers of unclumped cells (high density (1 × 105/60 mm); low density, (2 × 104/60 mm) of the indicated passage were seeded in duplicate in 0.367% agar. Total colony numbers were scored after 21 days. Contiguous clusters of >50 cells were considered colonies. Representative colonies were photographed as shown in B. Bar = 100 μm. C. H&E stained sections of tumors, 21 days after subcutaneous injection of NEK1 -/- RTEs into kat2J mice. Cy = cyst, arrows = abnormal nuclei. Bars = 100 μm. D. p19ARF expression in two distinct NEK1 -/- cell lines at different passages in culture, and in wild type (NEK1 +/+) cells at passage 7. Alpha tubulin expression served to control for protein loading in each lane.