Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: Regulation of PBX3 expression by androgen and Let-7d in prostate cancer

Figure 2

Androgen receptor independent regulation of PBX3 by R1881. A) LNCaP cells were pre-treated for 3 days with medium containing 10% CSS. Cells were then stimulated with 10-10M R1881 (R1881) and/or 10-8M bicalutamide (R1881 + Bic or Bic) or left untreated (CSS) for 4 days. Total protein extracts were analyzed by Western blotting using an anti-PBX3 antibody and anti-ERK 1/2 as loading control. Results of densitometric analysis of the PBX3 band relative to CSS are shown in the histogram. B) LNCaP cells were transfected with a small interfering RNA against androgen receptor (siRNA AR) and analyzed for PBX3 expression 48 hours post-transfection. A non-specific small interfering RNA (siRNA Ctr.) was used as control. Representative Western blots probed with anti-PBX3-, anti-AR-, anti-NKX3.1- and anti-α-tubulin antibodies are shown in the figure. The histograms depicts the results from densitometric analyses of PBX3 and AR relative to siRNA Ctr. Data were obtained from three independent experiments and are presented in the histograms as mean ± SD (n = 3). A t-test (paired two samples of mean) was performed and a two-tailed p-value < 0, 05 is indicated with a *.

Back to article page