Figure 6
From: Lipid phosphate phosphatase-3 regulates tumor growth via β-catenin and Cyclin-D1 signaling
Forced expression of LPP3 potentiates SW480 tumor growth in the xenograft assay. (A) pLNCX2-based retroviral constructs expressing vector alone, hLPP1, hLPP2, hLPP3 or mLpp3 cDNAs. (B) The comparable levels of hLPP1, hLPP2, hLPP3 and mLpp3 proteins were confirmed by western blotting with anti-HA antibody. (C) Xenograft assay: there was minimal tumor growth after 14 and 28 days in mice receiving LPP3-deficient SW480 cells (2 × 104 tumor cells/site) that expressed vector alone, hLPP1, or hLPP2 constructs. In contrast, tumor volume was significantly increased in mice receiving SW480 cells that expressed hLPP3 or mLpp3 (n = 12, ¥ P < 0.01 vs. hLPP1 group; n = 12, ¶ P < 0.005 vs. hLPP1 group) at 14 and 28 days.