Figure 5
From: IκB kinases increase Myc protein stability and enhance progression of breast cancer cells
IKKα and IKKβ increase tumorigenesis and invasiveness of MCF7 cells. (A) MTT assay was used to analyze the growth rate of indicated cells. Each data represents mean ± SD calculated from three independent experiments, and there are four wells for each point in a single experiment. (B) Assay of colony-forming ability in soft-agar. Each experiment have three 60 mm dishes and each data represents mean ± SD calculated from two independent experiments. (C) Assay of invasive ability of indicated cells. Each experiment have three trans-wells and each data represents mean ± SD calculated from two independent experiments. (D) Western blot analysis of the expression of cyclin D1 and twist protein in the indicated cells.