Concerted action of miR-15a/16 and miR-34a on individual mRNA targets. (A) mRNA levels of targets common to both miRNAs. H2009 cells were transfected with pre-miR-15a/16 or pre-miR-34a alone or co-transfected with both pre-miRNAs together (pre-miR-mix) at concentrations as indicated in the figure. (B) Expression level of targets unique to miR-15a/16 or miR-34a. Analysis was performed as described in the legend to Fig. 2C (n = 3). *, p < 0.05.