Figure 1
From: Natriuretic Peptide Receptor A as a Novel Target for Prostate Cancer
NPRA expression in tumor cells. (A) Immunoblot analysis demonstrating specificity of anti-NPRA antibody. Cell lysates were subjected to SDS-PAGE/immunoblot analysis with rabbit polyclonal antibody specific for human NPRA (top) and beta-actin (bottom). For the competition assay, lane 5 was incubated with NPRA-antibody adsorbed with NPRA peptide (20 μg/ml). (B) Western blotting for NPRA expression. β-actin expression was used as loading control. (C) Total RNA of indicated cell lines was analyzed for NPRA by real time PCR. NPRA expression was normalized with respect to β-actin. (D) Whole cell lysates of TRAMP-C1, C2, C3, CaP2 and P2 cells were analyzed by western blotting for NPRA. (E) ANP expression in PCa cells. Cells were cultured in media containing 0.5% fetal bovine serum for 48 hrs and the culture supernatants were analyzed for ANP by enzyme immunoassay kit (Phoenix Pharmaceuticals).