Figure 4
From: Natriuretic Peptide Receptor A as a Novel Target for Prostate Cancer
NPRA depletion inhibits MIF expression. (A) SuperArray analysis of prostate tissues of NPRA-KO and WT C57BL/6 mice. The relative expression level of genes that are altered in the prostate tissues of NPRA-KO vs. WT is shown. (B-C) Cells were transfected with iNPRAs. Whole cell lysates were extracted 72 hrs after transfection and examined for NPRA and MIF by western blotting. TRAMP-C1 cells were transfected with pshNPRAs or empty vector (B); PC3 cells were transfected with phNP73-102 plasmid or pVAX plasmid (C). (D) Relative band intensity for NPRA and MIF expression in Fig. 4C is shown.