Figure 2
From: Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH
Silencing of Siah2 or POSH enhances caspase activity in response to TRAIL. a: qRT-PCR assessment of Siah2 and POSH targeting in PC-3 and PPC-1 cells 24 hours after siRNA transfection. b: Caspase-8 activity in PC-3 cells transfected with siRNA specific for Siah2 or POSH for 72 hours and treated with TRAIL (100 ng/mL) for 2 and 4 hours. c: Caspase-3/7 activity in PC-3 cells treated as in (B) for 24 hours. Statistical comparison was made using a two-tailed Student's t-test. **P < 0.01, ***P < 0.001.