Figure 2
From: Effects of 1α,25 dihydroxyvitamin D3 and testosterone on miRNA and mRNA expression in LNCaP cells
The effect of T and 1,25(OH) 2 D 3 on mRNA expression in LNCaP cells. LNCaP cells were plated as described in Material and Methods, and treated with 5 nM T and 100 nM 1,25(OH)2D3 alone or in combination for 48 h. Total RNA was extracted and interrogated on Nimblegen-HG18-4plex arrays. Three independent replicates of each treatment were used for the analysis. Panel A: hierarchical clustering of the expression array data using the GeneSpring GX10 software. Panel B: Venn diagram analysis of the microarray data (Green: 1,25(OH)2D3-moduated, Orange: T-modulated, Blue: synergistically modulated genes by T and 1,25(OH)2D3. Panel C: Analysis of Selected Gene Ontologies Modulated by T and 1,25(OH)2D3 in LNCaP Cells. Functional annotation of each gene was assigned based on DAVID Bioinformatics Resources 2008 (NIAID). Magenta: gene up regulated by treatment; Green: down regulated by treatment; No shape outline: genes modulated by either T or 1,25(OH)2D3; dashed outline with white text: 1,25(OH)2D3 or T modulation is enhanced by the presence of T or 1,25(OH)2D3 respectively (synergy); solid outline with white text: additive effect of T and 1,25(OH)2D3 on mRNA levels; solid outline with black text: synergistic effect of T and 1,25(OH)2D3 on mRNA levels. Genes reported to be ion binding or ion channels are indicated (Ca2+: yellow, Cl-; blue, K+: purple, Na+: blue, Zn2+: blue).