Figure 6
From: Effects of 1α,25 dihydroxyvitamin D3 and testosterone on miRNA and mRNA expression in LNCaP cells
The effect of T and 1,25(OH) 2 D 3 on miRNA expression in LNCaP cells. Cells were plated as described in Material and Methods, and treated with 5 nM T and 100 nM 1,25(OH)2D3 alone or in combination for 48 h. Total RNA was extracted and interrogated on Agilent Human miRNA v3 arrays. Three independent replicates were used for the analysis. Panel A: hierarchical clustering of the miRNA array data using the GeneSpring GX10 software. Panel B: Gene Ontology analysis of mRNA targets of miRNAs, predominantly focused on those that are targeted at the 3'UTR and affect the transcript stability. The inverse correlations of miRNAs targeted mRNAs and their associated Gene Ontology grouping are summarized and color coded. Magenta: gene up regulated by treatment; Green: down regulated by treatment; No shape outline: genes modulated by either T or 1,25(OH)2D3; dashed outline with white text: 1,25(OH)2D3 or T modulation is enhanced by the presence of T or 1,25(OH)2D3 respectively (synergy); solid outline with white text: additive effect of T and 1,25(OH)2D3 on mRNA levels; solid outline with black text: synergistic effect of T and 1,25(OH)2D3 on mRNA levels. Genes reported to be ion binding or ion channels are indicated (Ca2+: yellow, Zn2+: blue). Different font size of miRNAs ranks the effect of miRNAs by the number of concordant mRNA targets in LNCaP cells.