Figure 2
From: Targeting oncogenic miR-335 inhibits growth and invasion of malignant astrocytoma cells
Effects of miR-335 on viability and invasion in C6 astrocytoma cells. (A) Dose-dependent effect of miR-335 mimics on cell viability. C6 cells were transfected with miR-335 mimics or negative control mimics (NC) for 72 h at the indicated concentrations. (B) Time-dependent effect of miR-335 mimics on cell viability. C6 cells were transfected with 50 nM miR-335 mimics for the indicated times. (C, D) Colony formation was observed at the presence of miR-335 mimics (C up-panel) or antagomir-335(C down-panel). (E, F) Cell invasiveness was detected by transwell invasion assay. C6 cells were transfected with 50 nM miR-335 mimics (E up-panel) or 100 nM antagomir-335 (E down-panel) or their counterpart negative controls. Graph is the representative of three independent experiments. (G) Dose-dependent effect of antagomir-335 on cell viability. C6 cells were transfected with antagomir-335 or antagomir-NC for 72 h at the indicated concentrations. (H) Time-dependent effect of antagomir-335 on cell viability. C6 cells were transfected with 100 nM antagomir-335 for the indicated times. (I) Cell viability was measured with MTT assay. Rat normal astrocytes and C6 astrocytoma cells were transfected with 100 nM antagomir-335 or antagomir-NC for 72 h. Data represent the means ± SD. Statistical differences compared with the controls are given as *, P < 0.05; **, P < 0.01; ***, P < 0.001. Original magnification in (E), 200 ×.