SiDaam1 mimics the oncogenic effects of miR-335 in C6 cells. (A) SiDaam1 efficiently inhibited the expression of DAAM1. Western blot was used to detect the expression of DAAM1 72 h after transfection with 50 nM siDaam1 or miR-335 mimics in C6 cells. (B) Cell viability was detected by MTT assay. (C) Effect of siDaam1 or miR-335 transfection on colony formation. (D) Morphologic alteration (up-panel) and phallotoxins stained actin rearrangement (down-panel). Cells transfected with siDaam1 or miR-335 adopted a stellate morphology and collapsed their actin stress fibers together with increased membrane ruffling. (E) Cell invasiveness was detected by transwell invasion assay. C6 cells were transfected with 50 nM siDaam1 or miR-335 mimics for the indicated times. Results represent the means ± SD for three repeats. (*, p < 0.05;**, p < 0.01). Original magnification in (D up-panel; E), 200 ×; (D down-panel), 630 ×.