Figure 3

Silencing of HOXD9 gene decreases proliferation of U87 glioma cells. (A) Analysis of HOXD9 gene expression by qRT-PCR 2 days after siRNA transfection. The graphs show the average of two independent experiments. For the graphs, the data are complied from three independent experiments. * *, P < 0.01. Error bars indicate ± S.D. (B) Growth suppression of U87 glioma cells after HOXD9 siRNA transfection. siRNA-transfected cells were counted by trypan blue exclusion. Data are representative of three independent experiments. *, P < 0.05. Error bars indicate ± S.D. (C) Cell cycle alteration by HOXD9 siRNA-1 in U87 glioma cells 3 days after siRNA treatment. Results of flow cytometry analysis using Vybrant DyeCycle Violet dye are shown. Data are representative of three independent experiments. (D) 3 weeks after siRNA transfection, colonies stained with MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] were counted. For the graphs, the data are complied from three independent experiments. *, P < 0.05. Error bars indicate ± S.D.